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目的:观察食盐对自发性高血压大鼠(SHR)及正常血压大鼠(WKY)心肌纤维化的影响,并探讨其可能机制。方法:SHR和WKY各20只分别分为两组:①高盐饮食组(SHRSL,n=10和WKYSL,n=10)饮用含2％NaCl溶液;②正常盐饮食组(SHRNS,n=10和WKYNS,n=10)饮用不含NaCl清水,共饲养6周。VG染色和图像处理观察心肌纤维化情况,放免测定心肌局部内皮素-1(ET-1)含量。结果:①SHRSL组心肌间质胶原百分比(CVF)及血管周围胶原面积与血管腔面积百分比(PVCA)显著高于SHRNS(P<0.01),WKYSL组的CVF、PVCA明显高于WKYNs组(P<0.05);②SHRSL组心肌局部ET-1含量显著高于SHRNS组(P<0.01),WKYSL组与WKYNS组心肌局部ET-1含量无差别(P>0.05)。结论:高盐饮食可引起SHR及WKY心肌纤维化,盐负荷后SHR心肌局部ET-1含量的增加可能是SHR心肌纤维化加重的原因之一。
Objective: To observe the effect of salt on myocardial fibrosis in spontaneously hypertensive rats (SHR) and normal blood pressure rats (WKY) and to explore its possible mechanism. Methods: Twenty SHR and WKY were divided into two groups: ① high salt diet group (SHRSL, n = 10 and WKYSL, n = 10) drinking 2% NaCl solution; ② normal saline diet group And WKYNS, n = 10) drinking water without NaCl for a total of 6 weeks. VG staining and image processing were used to observe myocardial fibrosis, and the content of endothelin-1 (ET-1) in myocardium was determined by radioimmunoassay. Results: ① The percentages of CVF and PVCA in SHRSL group were significantly higher than those in SHRNS group (P <0.01). The CVF and PVCA in WKYSL group were significantly higher than those in WKYNs group (P <0.05) ); ② The content of ET-1 in myocardium of SHRSL group was significantly higher than that of SHRNS group (P <0.01). There was no difference in myocardial ET-1 between WKYSL group and WKYNS group (P> 0.05). Conclusion: High salt diet can induce myocardial fibrosis of SHR and WKY. The increase of local ET-1 in SHR after salt loading may be one of the reasons of myocardial fibrosis in SHR.