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目的:建立GC法同时测定复方牙痛酊中樟脑和乙酸龙脑酯的含量。方法:采用DB-WAX毛细管柱(30 m×0.25 mm×0.25μm),氢火焰离子检测器(FID);以萘为内标;进样口温度230℃,检测器温度250℃,程序升温(初始温度105℃,保持3min,以3℃·min-1的速率升至140℃,再以100℃·min-1的速率升至210℃,保持5 min),分流进样,分流比2.0∶1,进样量1μL。结果:樟脑、乙酸龙脑酯浓度分别在1.81~27.18μg·m L-1和5.53~82.95μg·m L-1范围内呈良好的线性关系(r=0.999 9),平均回收率(n=9)分别为103.9%和105.2%。结论:本方法可用于测定复方牙痛酊中樟脑和乙酸龙脑酯的含量。
Objective: To establish a GC method for simultaneous determination of camphor and butyronate in compound toothache tincture. Methods: DB-WAX capillary column (30 m × 0.25 mm × 0.25 μm) and hydrogen flame ionization detector (FID) were used. The internal standard of naphthalene was as follows: inlet temperature was 230 ℃, detector temperature was 250 ℃, temperature programmed The initial temperature was maintained at 105 ° C for 3 min, the temperature was raised to 140 ° C at a rate of 3 ° C · min-1, and the temperature was raised to 210 ° C at a rate of 100 ° C · min-1 for 5 min. 1, injection volume 1μL. Results: The concentrations of camphor and borneol were linear in the range of 1.81 ~ 27.18μg · m L-1 and 5.53 ~ 82.95μg · m L-1 respectively (r = 0.999 9). The average recoveries (n = 9) were 103.9% and 105.2% respectively. Conclusion: This method can be used to determine the content of camphor and borneol ester in compound toothache tincture.