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采用差速离心分离技术与超热中子活化分析法 (ENAA) ,测定了大鼠肝脏亚细胞组分 (细胞核、线粒体、溶酶体、微粒体、胞液 )中As的质量分数 ,并对标准参考物质作对照分析。分析结果表明 :As在各亚细胞组分中并非均匀分布。As在微粒体中的质量分数最高 ,在细胞核中最低。用t检验比较了在中毒组大鼠和正常组大鼠肝脏中各亚细胞组分中As含量的差别 ,以及中毒组大鼠肝亚细胞组分之间的差异。发现上述两组肝亚细胞组分中As存在极显著性差异 (P <0 0 0 1)。在中毒组肝亚细胞组分中 ,微粒体中As的含量与胞液无显著性差异 (P >0 0 5 ) ,但与细胞核、线粒体、溶酶体存在明显的差异 (P <0 0 5 )。
The mass fraction of As in rat liver subcellular fractions (nucleus, mitochondria, lysosome, microsome and cytosol) was measured by differential centrifugation and hyperthermic thermal neutron activation assay (ENAA) Standard reference material for comparative analysis. The results show that As is not evenly distributed among the subcellular fractions. As in the microsomes in the highest mass fraction, the lowest in the nucleus. The differences of As content in subcellular fractions in the liver of poisoning rats and normal rats and the difference of hepatic subcellular fractions between rats in poisoning group and rats in poisoning group were compared by t-test. There was a significant difference in As between the two groups of hepatocyte subgroups (P <0.01). In the hepatocyte subgroups of the poisoning group, the content of As in the microsome was not significantly different from that in the cytosol (P> 0.05), but significantly different from that in the nucleus, mitochondria and lysosome (P <0.05 ).