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目的 探索异基因外周血造血干细胞移植治疗急性再生障碍性贫血 (再障 )的疗效及其长期造血重建。方法 1例急性再障患者 ,30岁 ,供者为其胞弟 ,HLA配型完全相合。动员方案 :G CSF 2 5 0 μg/d× 6d。预处理方案 :环磷酰胺 (CTX) 5 0mg·kg-1·d-1× 4d ,抗胸腺细胞球蛋白 (ATG)2 0mg·kg-1·d-1× 2d ,移植有核细胞数MNC 8.97× 10 8/kg ,CD3 4+ 细胞 9.2 0× 10 6/kg ,CFU GM11.2 0× 10 5/kg。结果 移植后 18天造血重建。三个微卫星位点PCR监测显示移植后 18天为混合型嵌合 ,2 5天后为持续完全供者型嵌合。未发生急、慢性移植物抗宿主病 (GVHD) ,随访 2 40d血常规、骨髓象正常。结论 异基因外周血造血干细胞移植可有效治疗急性再障 ,本例造血重建迅速 ,移植相关并发症少
Objective To explore the efficacy of allogeneic peripheral blood stem cell transplantation in the treatment of acute aplastic anemia (AA) and its long-term hematopoietic reconstitution. Methods A case of acute aplastic anemia, 30 years old, donor for their younger brother, HLA matching completely matched. Mobilization regimen: G CSF 250 μg / d × 6d. Pretreatment regimen: CTX 50 mg · kg-1 · d-1 × 4d, anti-thymocyte globulin (ATG) 20 mg · kg-1 · d-1 × 2d, the number of transplanted nucleated cells MNC 8.97 × 10 8 / kg, CD3 4+ cells 9.2 × 10 6 / kg, and CFU GM11.2 × 10 5 / kg. Results 18 days after transplantation, hematopoietic reconstructions. PCR monitoring of the three microsatellite loci showed mixed chimerism 18 days after transplantation and continued complete donor chimerisation after 25 days. No acute and chronic graft-versus-host disease (GVHD) was followed up for 2 40 days and the bone marrow was normal. Conclusions Allogeneic peripheral blood stem cell transplantation can effectively treat acute aplastic anemia. In this case, hematopoietic reconstitution is rapid with fewer complications related to transplantation