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目前用于光化学疗法的补骨脂素如8-MOP具有二个反应位置,在UVA的作用下,可与细胞核的DNA发生交联结合,形成单加合物与双加合物,不易为细胞修复机制所去除,因此不仅能引起许多生物的细胞突变,而且可使小鼠致癌。因而有必要寻求其他具有光敏作用,而又无致癌可能的化合物以代替目前应用的补骨脂素。3-羰乙氧基补骨脂素(3-Carbethoxypsoralen,3-CPs)仅有一个反应位,在UVA的作用下,与细胞DNA只形成单加合物。实验证明,对酵母菌DNA的光亲合力(photo-affinity)较8-MOP约高16倍,但其杀灭酵母菌的能力却较8-MOP低5～6倍。提示其与DNA形成的单加合物较8-MOP与DNA形成的加合物更易为修复机制所去除。由于3-CPs主要是
At present, psoralen used for photochemotherapy, such as 8-MOP, has two reaction sites. Under the action of UVA, it can cross-link with the DNA of the cell nucleus to form mono- and di-adducts. It is not easy to be a cell. The repair mechanism is removed, so not only can cause mutations in many organisms, but also can cause cancer in mice. Therefore, it is necessary to seek for other compounds that have a photosensitizing effect and no carcinogenic potential to replace the currently used psoralen. 3-Carbethoxypsoralen (3-CPs) has only one reaction site and forms only a single adduct with cellular DNA under the action of UVA. Experiments have shown that the photo-affinity of yeast DNA is about 16 times higher than that of 8-MOP, but its ability to kill yeast is 5-6 times lower than that of 8-MOP. It is suggested that the mono-adduct formed with DNA is more easily removed by the repair mechanism than the adduct formed by 8-MOP and DNA. Because 3-CPs are mainly