论文部分内容阅读
目的:观察健足颗粒(加减防己黄芪汤)对糖尿病皮肤溃疡大鼠血管内皮功能的影响。方法:选用SD雄性大鼠90只,随机选取6只为正常对照组,其余大鼠以尾静脉注射四氧嘧啶40mg/kg,筛取符合糖尿病诊断标准的大鼠用于建立糖尿病皮肤溃疡模型,并随机分成6组,模型对照组、健足颗粒干预的高、中、低剂量组,中药对照组、西药对照组。实验结束后断头取血检测NO、ET-1含量以观察健足颗粒对血管内皮功能的影响。结果:与模型组比较,各治疗组大鼠血清NO含量均有不同程度升高,而血浆ET-1含量却有不同程度的降低,其中以健足颗粒高剂量组变化最明显(P<0.001),其次为中剂量(P<0.01),低剂量、中药对照组次之(P<0.05),西药对照组也有轻度改变,但无显著统计学意义(P>0.05)。健足颗粒各剂量组的调节作用与剂量呈现相关性。结论:健足颗粒可通过升高血清NO含量、降低血浆ET-1水平来改善血管内皮功能,有效防治糖尿病皮肤溃疡发生发展。
Objective: To observe the effect of Jianzu granule (add and subtract anti-Jihuang Qi Decoction) on vascular endothelial function in diabetic skin ulcer rats. Methods: Ninety male Sprague Dawley rats were randomly selected. Six rats were randomly selected as normal control group. All the rats were injected alloxan 40mg / kg into caudal vein to screen diabetic rats for diabetic skin ulcer model. The rats were randomly divided into 6 groups: model control group, high, medium and low dose intervention group of Jianzu granule, traditional Chinese medicine control group and western medicine control group. At the end of the experiment, the levels of NO and ET-1 in blood were measured to observe the effect of Jian-Zu-Particle on endothelial function. Results: Compared with the model group, the content of serum NO in each treatment group increased to some extent, but the content of ET-1 in plasma decreased to some extent. The most obvious change was in the Jianzheng granule high dose group (P <0.001) ), Followed by medium dose (P <0.01), low dose, followed by traditional Chinese medicine control group (P <0.05), western medicine control group also mildly changed, but not statistically significant (P> 0.05). The regulation effect of Jianzi granule in each dose group showed dose dependence. Conclusion: Jianzu granule can improve vascular endothelial function by increasing serum NO level and decreasing plasma ET-1 level, effectively preventing and treating diabetic skin ulcers.