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本文报道小麦花药和幼穗可一步培养成苗的研究结果。小麦幼穗在添加低于0.5mg/l2,4-D 和高于1.0mg/16-BA 的 C17培养基上,先暗培养约15天,再转到光培养室培养,在同一培养基土可分化出大量植株。小麦花培,取花粉发育时期适宜的幼穗,立即进行约5天的适当低温处理,取花药用碘—碘化钾液压片染色,进行花粉发育时期的镜检,然后选取花粉粒处于单核后期的花药接种培养,培养基为含有低于0.5mg/l 2,4-D 和激动素的 C17培养基,先暗培养约20天,再光培养,可在同一培养基上分化出花粉植株。
This paper reports the results of wheat seedlings and anther culture in one step. Wheat young ears were cultured in C17 medium supplemented with less than 0.5mg / l 2,4-D and more than 1.0mg / 16-BA for about 15 days before being transferred to a light culture chamber for culture in the same medium Can differentiate a large number of plants. Wheat flower culture, taking the appropriate ear during the pollen development stage, immediately for about 5 days of low temperature treatment, taking anther iodine - potassium iodide tablet dyeing, microscopic examination of pollen development period, and then select the pollen grains in the mononuclear late The anther culture was inoculated with C17 medium containing less than 0.5 mg / l 2,4-D and kinetin, dark-cultured for about 20 days and then cultured again to differentiate pollen plants on the same medium.