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目的 研究 2 1 氨基类固醇 (Lazaroid ,U 75 412E)在体外对石英毒性的抑制作用。方法 观察体外培养的肺泡巨噬细胞 (AM)释放H2 O2 、乳酸脱氢酶 (LDH)、超氧化物歧化酶 (SOD)和β N 乙酰氨基葡萄糖苷酶 (NAG)的水平 ,评价U 75 412E对石英细胞毒性的消除能力 ;电子自旋共振俘获法评价U 75 412E清除石英催化H2 O2 产生·OH的作用 ;噻唑蓝比色法比较U 75 412E对矽肺大鼠肺泡AM促成纤维细胞 (FB)生长活性的抑制作用。结果 在U 75 412E作用下 ,与石英共孵的肺泡AM释放H2 O2 、SOD、LDH的水平显著下降 ,40 μmol/LU 75 412E组各值分别为 (0 .16± 0 .0 4)nmol/L、(48.6± 2 2 .2 ) μg/L、(33.0± 4.6 )U/L ,而阳性对照组分别为 (0 .6 3± 0 .0 7)nmol/L、(117.5± 30 .3) μg/L、(6 0 .3± 5 .5 )U/L。在Fenton反应体系中 ,石英催化H2 O2 生成·OH ,平均峰值为 (5 3.2 5± 2 .3)mm ;当与U 75 412E共存时 ,反应体系中的·OH水平显著下降 ,平均峰值为 (2 8.4±1.3)mm。矽肺或受石英攻击的肺泡AM培养液促进了NIH 3T3细胞增殖 (平均指数 :77.8± 8.0、85 .9± 7.0 ) ,U 75 412E显著抑制了这一活性 (平均指数 :5 8.0± 15 .4、5 2 .6± 11.8)。结论 U 75 412E具有较强的清除·OH、保护AM抵御石英细胞毒性、抑?
Objective To study the inhibitory effect of 2 1 amino steroids (Lazaroid, U 75 412E) on quartz toxicity in vitro. Methods The level of H2O2, lactate dehydrogenase (LDH), superoxide dismutase (SOD) and β-N-acetylglucosaminidase (NAG) released from alveolar macrophages (AM) On the cytotoxicity of quartz cells. The electron spin resonance capture method was used to evaluate the effect of U 75 412E on quartz · OH · 2H 2 O · OH. Thiazolyl blue colorimetric assay was used to compare the effect of U 75 412E on alveolar AM-fibroblasts (FB) Inhibition of growth activity. Results Under the action of U 75 412E, the level of H2O2, SOD and LDH released from alveolar AMs incubated with quartz decreased significantly. The values of 40 μmol / LU 75 412E were (0.16 ± 0.04) nmol / L, (48.6 ± 22.2) μg / L and (33.0 ± 4.6) U / L respectively, while the positive control group was (0.63 ± 0.07) nmol / L and (117.5 ± 30.3 ) μg / L, (6 0 .3 ± 5 .5) U / L. In the Fenton reaction system, the average peak value of H2O2 was (5 3.2 5 ± 2 .3) mm when H2O2 was catalyzed by quartz. When · U 75 412E coexisted, the · OH level in the reaction system decreased significantly with an average peak value of ( 2 8.4 ± 1.3) mm. Silicosis or quartz-challenged alveolar AM cultures promoted proliferation of NIH 3T3 cells (mean index: 77.8 ± 8.0, 85.9 ± 7.0) and U 75 412E significantly inhibited this activity (mean index: 5 8.0 ± 15.4 , 52.6 ± 11.8). Conclusion U 75 412E has a strong ability to scavenge OH and protect AM from quartz cytotoxicity.