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对细胞因子基因修饰瘤苗的研究表明,IL-2、IL-4、IFN-γ、GM-CSF等一系列细胞因子基因以不同载体转入肿瘤细胞制成瘤苗后皮下免疫小鼠,均可增强机体抗肿瘤免疫力,其机制可能是由于瘤苗分泌的细胞因子促进了免疫细胞对肿瘤抗原的识别、提呈及对肿瘤细胞的杀伤能力.有文献报道,逆转录病毒介导的GM-CSF和IL-4共同转染瘤苗可以有效激发机体抗肿瘤免疫力,为探讨不同途径瘤苗免疫后机体的免疫反应,我们采用皮下、腹腔、脾内、静脉四种途径接种GM-CSF、IL-4基因双重转染的小鼠红白血病细胞FBL-3瘤苗,发现免疫途径不同,所激发的免疫应答类型不同,诱导机体生成的免疫力不同,提示某些瘤苗应用时应选择适当的免疫途径.
The study of cytokine gene modified tumor vaccines showed that IL-2, IL-4, IFN-γ, GM-CSF and other cytokine genes were transferred into tumor cells with different vectors to make subcutaneously immunized mice with tumor vaccines. It can enhance the body’s anti-tumor immunity, the mechanism may be due to cytokines secreted by tumor vaccines to promote the recognition of tumor cells antigen, presentation and killing ability of tumor cells. There are reports in the literature, retrovirus-mediated GM - CSF and IL-4 co-transfection of tumor vaccines can effectively stimulate the body’s anti-tumor immunity. To investigate the immune responses of tumor vaccines after different routes, we used subcutaneous, intraperitoneal, intrasplenial, and intravenous routes to inoculate GM-CSF. The FBL-3 tumor vaccine, a mouse erythroleukemia cell double-transfected with IL-4 gene, was found to have different immune pathways, different types of immune responses were elicited, and different immune responses were generated, suggesting that certain tumor vaccines should be selected for application. Appropriate immunization route.