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目的了解小球藻的安全性。方法以2.0、4.0、8.0g/kg·BW 3个剂量给予大鼠经口摄入30d,观察体重、进食量,食物利用率、血常规、血生化以及外观及脏器的病理变化。结果试验结束时,个别剂量组大鼠的血清谷丙转氨酶、肌酐、尿素、白蛋白、总蛋白和甘油三酯等指标与对照组比较,差异有统计学意义(P<0.05),但均在本实验室的正常值范围内。其它剂量组大鼠每周体重、体重增重、进食量、食物利用率及血红蛋白、红细胞计数、白细胞计数和白细胞分类、血清谷丙转氨酶、谷草转氨酶、尿素、肌酐、总胆固醇、甘油三酯、血糖、总蛋白、白蛋白、脏/体比值与对照组比较差异均无统计学意义,高剂量组和对照组仅个别动物出现了肝小叶空泡变、汇管区炎症细胞浸润、胃萎缩、肠炎症、肾小管管型,两组病变发生率差异均无统计学意义,两组其他器官均未出现特异性病变。结论小球藻大鼠30d毒性研究,大鼠未见异常。
Objective To understand the safety of chlorella. Methods The rats were orally administrated with 3 doses of 2.0, 4.0 and 8.0 g / kg · BW for 30 days. The body weight, food intake, food utilization rate, blood routine, blood biochemistry and pathological changes of viscera were observed. Results At the end of the experiment, the serum alanine aminotransferase, creatinine, urea, albumin, total protein and triglyceride were significantly decreased in the individual dose groups compared with the control group (P <0.05) The normal range of the laboratory. The body weight, body weight gain, food intake, food utilization and hemoglobin, erythrocyte count, white blood cell count and white blood cell classification, serum alanine aminotransferase, aspartate aminotransferase, urea, creatinine, total cholesterol, triglyceride, Blood glucose, total protein, albumin and somatic / visceral ratio were not significantly different from those of the control group. In the high-dose group and the control group, only a few of the animals showed hepatic lobule vacuolar changes, inflammatory cell infiltration in portal area, gastric atrophy and enteritis Symptoms, tubular tubular type, the incidence of lesions was no significant difference between the two groups, the other two groups did not appear specific lesions. Conclusion Chlorella rats 30d toxicity studies, no abnormalities in rats.