论文部分内容阅读
目的通过慢病毒载体转染将SOCS1基因导入未成熟树突状细胞(DC)构建免疫耐受DC,并研究该耐受DC的免疫表型、刺激增殖等功能状态。方法根据Gene Bank报道的细胞因子信号转导抑制分子1(SOCS1)mRNA序列(NM_009896.2)合成SOCS1基因,构建及鉴定过表达SOCS1基因的慢病毒载体,并体外转染未成熟DC;通过流式分选技术分析CD80、CD86和MHCⅡ等因子表达确定细胞表型;经
OBJECTIVE: To construct the immunocompromised DC by introducing the SOCS1 gene into immature dendritic cells (DC) by lentiviral vector transfection and to study the functional status of the immunocompromised DC that is resistant to DC and to stimulate proliferation. Methods The SOCS1 gene was synthesized according to the cDNA sequence of cytokine signaling suppressor molecule 1 (SOCS1) reported by Gene Bank (NM_009896.2), and the lentiviral vector overexpressing SOCS1 gene was constructed and identified. Immature DCs were transfected by flow cytometry Sorting technology analysis of CD80, CD86 and MHC Ⅱ and other factors to determine the cell phenotype;