论文部分内容阅读
目的了解高致病性H5N1禽流感病毒在浙江省义乌市职业暴露人群中的感染变化状况及其在环境中的分布情况。方法马血球血凝抑制试验(HI)检测高致病性H5N1禽流感病毒抗体,实时荧光定量PCR检测A型流感病毒。结果在105份职业暴露人群血清标本中检出H5N1抗体阳性标本12份,总检出率为11.43%。男、女性别间H5N1抗体阳性率差异无统计学意义(χ2=0.064,P>0.05);家禽规模养殖场(户)和家禽散养户两类职业暴露人群间H5N1抗体阳性率差异无统计学意义(χ2=1.304,P>0.05);226份环境标本中检出H9亚型禽流感病毒1份,未检出H5亚型。结论义乌市职业暴露人群中存在高致病性H5N1禽流感病毒隐性感染的情况或有个别轻症病例的可能,环境中存在禽流感病毒的污染,未发现高致病性H5N1禽流感病毒污染的证据,应加大监测力度。
Objective To investigate the infection status of H5N1 avian influenza virus in occupationally exposed population in Yiwu City, Zhejiang Province and its distribution in the environment. Methods The HI antibody was used to detect H5N1 avian influenza virus (H5N1) and the influenza A virus was detected by real-time fluorescence quantitative PCR. Results 12 positive samples of H5N1 antibody were detected in serum samples from 105 occupational exposure groups, with a total detection rate of 11.43%. There was no significant difference in the positive rate of H5N1 antibody between male and female (χ2 = 0.064, P> 0.05). There was no significant difference in the positive rate of H5N1 between two groups of occupational exposure in poultry scale farms and poultry (Χ2 = 1.304, P> 0.05). One H22 subtype of H5N1 virus was detected in 226 environmental samples and no H5 subtype was detected. Conclusion There is a case of latent infection of H5N1 avian influenza virus among occupationally exposed people in Yiwu City or there may be some mild cases in which there is contamination of avian influenza virus in the environment and no contamination of highly pathogenic H5N1 bird flu virus The evidence should be increased monitoring efforts.