建立了高效液相色谱法拆分盐酸莫西沙星及其对映异构体,并定量测定对映异构体的含量。采用Zorbax SB-C18色谱柱,流动相为手性流动相添加剂溶液(含3 mmol/L硫酸铜与6 mmol/L L-苯丙氨酸的水溶液)-甲醇(78︰22),检测波长293 nm。盐酸莫西沙星与其对映异构体分离完全,对映异构体在0.1～2 g/ml的浓度范围内线性关系良好,平均回收率为98.6%,RSD为1.2%。 A HPLC method was established for the resolution of moxifloxacin hydrochloride and its enantiomers and the quantitative determination of enantiomers. Zorbax SB-C18 was used as the mobile phase. The mobile phase consisted of chiral mobile phase additive solution (containing 3 mmol / L copper sulfate and 6 mmol / L L-phenylalanine in water) -methanol (78:22) nm. The separation of moxifloxacin hydrochloride from its enantiomers was complete. The enantiomers had a good linear relationship in the concentration range of 0.1-2 g / ml with an average recovery of 98.6% and a RSD of 1.2%.