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由茄镰孢豌豆专化型(Fusarium solani f.sp.pisi,Fsp)引起的根腐病是豌豆(Pisum sativum)最重要的病害之一。研究不同地理来源Fsp的遗传多样性,对了解该菌的遗传背景及防治病害具有重要意义。本研究从血红丛赤壳交配群VI(Nectria haematococca MPVI)全基因组序列中筛选SSR位点,选取107个SSR位点设计引物,获得24对多态性引物。用24对多态性引物对不同地理来源的96个Fsp分离物进行遗传多样性分析,结果表明,24对引物共扩增出132个等位基因,变异范围为3-15,平均为5.5。基因多样性指数范围为0.4855-0.8264,平均为0.7038。供试的96个Fsp分离物可分为93个基因型。聚类分析表明,在相似性系数为0.8时,96个Fsp分离物被划分为10个组群。Fsp分离物的地理来源或致病性与SSR聚类结果无关。分子方差分析(AMOVA)结果表明,Fsp遗传变异主要存在于群体内,地理条件和生态区环境对Fsp遗传分化有显著影响。
Root rot caused by Fusarium solani f.sp.pisi (Fsp) is one of the most important diseases of pea (Pisum sativum). Studying the genetic diversity of Fsp from different geographical sources is of great significance to understand the genetic background of the bacteria and to prevent and cure diseases. In this study, SSR loci were screened from the whole genome sequence of Nectria haematococca VI (CVVI). 107 SSR loci were selected to design primers and 24 pairs of polymorphic primers were obtained. Twenty-four polymorphic primers were used to analyze the genetic diversity of 96 Fsp isolates from different geographical origins. The results showed that a total of 132 alleles were amplified by 24 pairs of primers, with a variation range of 3-15 with an average of 5.5. The genetic diversity index ranged from 0.4855 to 0.8264, with an average of 0.7038. The 96 tested Fsp isolates were divided into 93 genotypes. Cluster analysis showed that at the similarity coefficient of 0.8, 96 Fsp isolates were divided into 10 groups. The geographical origin or pathogenicity of Fsp isolates has nothing to do with the SSR clustering results. Molecular variance analysis (AMOVA) showed that the genetic variation of Fsp mainly existed in the population, and the geographical conditions and ecological environment had a significant impact on the genetic differentiation of Fsp.