论文部分内容阅读
细菌性病害给蘑菇栽培带来的危害较大,黄色单胞菌可感染蘑菇,使其子实体腐烂而死亡,直接影响到蘑菇的产量与质量。我们于1980年开始对此病进行了试验研究。材料和方法 (一) 病菌菌株分离:1980年12月20日,从感染菇床上采集的病菇,在无菌条件下,用组织分离的方法,切取菇体感病部位的组织块,于普通PDA斜面上,待组织块周围出现明显的菌落后,移植、分纯、扩大培养。 (二)制片染色方法:于载玻片滴0.85％
Bacterial diseases to the mushroom cultivation greater harm, yellow mushroom can infect mushrooms, so that its fruiting body decay and death, a direct impact on mushroom yield and quality. We started the disease in 1980 to conduct a pilot study. Materials and methods (I) Isolation of pathogenic bacteria: December 20, 1980, mushrooms collected from infected bed mushrooms, under sterile conditions, the method of tissue separation, cut the susceptible parts of mushroom tissue block, in the normal PDA Oblique surface, to be obvious around the block after the colonies, transplantation, pure points, expand training. (B) preparation staining method: 0.85% slide glass slides