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目的探讨不同塑化剂皮肤暴露对小鼠过敏性皮肤炎症的影响及其机制。方法将所有Balb/C小鼠随机均分为6组,每组10只。A组:动物不做任何处理;B组:生理盐水皮肤暴露+0.5%FITC致敏;C组:100 mg/(kg·d)的DIDP皮肤暴露+0.5%FITC致敏;D组:140 mg/(kg·d)DINP皮肤暴露+0.5%FITC致敏;E组:100 mg/(kg·d)的DIDP皮肤暴露+0.5%FITC致敏+MT:F组:140 mg/(kg·d)DINP皮肤暴露+0.5%FITC致敏+MT。各组动物给予相应药物,至第28 d处死,取脏器,测定耳肿胀程度、检测耳组织病理学变化、测定耳组织匀浆ROS、GSH、MDA水平。结果与A组比较,B组耳肿指数、双耳重量差、耳部组织细胞浸润和耳部组织结构破坏明显升高,差异具有统计学意义(P<0.05);与B组比较,C、D组耳肿指数、双耳重量差、耳部组织细胞浸润和耳部组织结构破坏明显升高,差异具有统计学意义(P<0.05);与C组比较,E组耳肿指数、双耳重量差、耳部组织细胞浸润和耳部组织结构破坏明显降低,差异具有统计学意义(P<0.05);与D组比较,F组耳肿指数、双耳重量差、耳部组织细胞浸润和耳部组织结构破坏明显降低,差异具有统计学意义(P<0.05);与A组比较,B组ROS、MDA明显升高,GSH明显降低,差异具有统计学意义(P<0.05);与B组比较,C、D组ROS、MDA明显升高,GSH明显降低,差异具有统计学意义(P<0.05);与C组比较,E组ROS、MDA明显降低,GSH明显升高,差异具有统计学意义(P<0.05);与D组比较,F组ROS、MDA明显降低,GSH明显升高,差异具有统计学意义(P<0.05)。结论皮肤暴露DIDP和D1NP,对小鼠过敏性皮肤炎症起到明显的佐剂作用,其可能原因是小鼠机体氧化应激过度活化形成的氧化损伤加重了炎症反应。
Objective To investigate the effects of different skin plasticizers on allergic skin inflammation in mice and its mechanism. Methods All Balb / C mice were randomly divided into 6 groups of 10. Group A: animals were treated without treatment; Group B: saline skin exposure + 0.5% FITC sensitization; Group C: DIDP skin exposure + 0.5% FITC sensitization at 100 mg / (kg • d); Group D: 140 mg / (kg · d) DINP skin exposure + 0.5% FITC sensitization; Group E: 100 mg / (kg · d) DIDP skin exposure + 0.5% FITC sensitization + MT: F group: 140 mg / (kg · d ) DINP skin exposure + 0.5% FITC sensitization + MT. Animals in each group were given the corresponding drugs, and the animals were sacrificed on the 28th day. The organs were removed and their ear swelling was measured. The pathological changes of the ears were detected. The levels of ROS, GSH and MDA in the ears were measured. Results Compared with group A, the index of ear swelling, the weight of binaural, the infiltration of ear cells and the destruction of the structure of ear were significantly increased in group B, the difference was statistically significant (P <0.05). Compared with group B, In group D, the index of ear swelling, the difference of ear weight, the infiltration of ear cells and the destruction of ear tissue were significantly increased (P <0.05). Compared with group C, the number of ear swelling index, ear (P <0.05). Compared with group D, the index of ear swelling, the difference of ear weights, the infiltration of ear cells and the percentage of ear tissue cells in group F were significantly lower than those in group D (P <0.05). Compared with group A, ROS and MDA in group B were significantly increased and GSH was significantly decreased (P <0.05), and the difference between group B and group B was statistically significant Compared with group C, ROS and MDA were significantly decreased and GSH was significantly increased in group C and group D, the difference was statistically significant (P <0.05). Compared with group C, ROS and MDA in group C and group D were significantly increased (P <0.05). Compared with group D, ROS and MDA in group F were significantly decreased and GSH was significantly increased (P <0.05). Conclusion The skin exposed DIDP and D1NP play an obvious adjuvant effect on allergic skin inflammation in mice. The possible reason is that the oxidative damage caused by over-activation of oxidative stress in mice aggravates the inflammatory reaction.