为了筛选出适宜长白9号种植的黑土、盐碱土的最佳配比,研究了5种土壤对长白9号抽穗后叶、穗、茎、鞘各器官渗透调节能力及膜损伤程度的影响。结果表明:黑土∶碱土=1∶1处理(C)游离氨基酸、脯氨酸及可溶性蛋白质含量高于其他配比土壤;黑土与碱土3∶1时,可溶性蛋白和可溶性糖含量与黑土基本一致,游离氨基酸与脯氨酸含量更接近于C处理;原盐碱土生长的长白9号抽穗后各器官的渗透调节物质积累相对较少,丙二醛含量与O2-·产生速率明显高于其他处理;抽穗至成熟,长白9号各器官渗透调节物质的积累顺序依次为叶>穗>茎>鞘,说明原盐碱土高Na+浓度和高pH值使长白9号渗透调节能力降低,膜损伤程度加速;黑土与碱土的比例达到1∶1或更高时,可有效调节土壤的Na+浓度和pH值,长白9号各器官的渗透调节能力提高,叶和穗两器官尤为明显。 In order to screen out the best ratio of black soil and saline-alkali soil planted in Changbai 9, the effects of five kinds of soil on the osmotic adjustment ability and membrane damage degree of leaves, spikes, stems and sheaths after heading of Changbai 9 were studied. The results showed that the contents of free amino acids, proline and soluble protein in black soil: alkaline soil = 1: 1 treatment were higher than those in other soil profiles. The contents of soluble protein and soluble sugar in black soil and alkaline soil were basically the same as those in black soil, Free amino acids and proline content were closer to that of C treatment. After salinity-alkaline soil was grown, Changbai 9 had less accumulation of osmolytes, malondialdehyde content and O2- production rate than those of other treatments. The order of accumulation of osmolytes in Changbai 9 organs was leaf> ear> stem> sheath, which indicated that the high Na + concentration and high pH value of the original saline-alkali soil decreased the osmotic adjustment ability of Changbai 9 and accelerated the damage of the membrane. When the proportion of alkaline earth reached 1: 1 or higher, the Na + concentration and pH of soil could be effectively regulated. The osmotic adjustment ability of all organs of Changbai 9 was enhanced, especially in leaves and spikes.