论文部分内容阅读
目的:研究细胞信号转导抑制因子1(SOCS1)基因对人口腔癌细胞增殖、周期及体外侵袭生物学功能的影响。方法:Western blotting及实时定量PCR验证人口腔癌细胞系KB中基因SOCS1的干扰效果;MTT法检测细胞增殖;应用Transwell侵袭小室模型观察口腔癌细胞的侵袭能力;流式细胞术分析细胞周期的分布情况。结果:SOCS1干扰片段显著降低口腔癌KB细胞SOCS1基因的m RNA及蛋白表达水平。抑制SOCS1表达后,细胞体外侵袭能力明显下降(P<0.05),且转染72 h后KB细胞数量较对照组显著减少(P<0.05);干扰KB细胞SOCS1基因表达,使G0/G1期细胞数目明显多于对照组(P<0.05),而S、G2/M期细胞数目显著少于对照组(均P<0.05)。结论:干扰人口腔癌细胞株KB中的SOCS1基因表达后,KB细胞增殖、体外侵袭能力减弱,细胞分裂受到抑制。
Objective: To investigate the effects of SOCS1 gene on the proliferation, cell cycle and biological function of human oral cancer cells in vitro. Methods: Western blotting and real-time quantitative PCR were used to verify the interference effect of SOCS1 in human oral cancer cell line KB. The cell proliferation was detected by MTT assay. The invasion ability of oral cancer cells was observed by Transwell invasion chamber model. The cell cycle distribution was analyzed by flow cytometry Happening. Results: The SOCS1 interference fragment significantly reduced the mRNA and protein levels of SOCS1 in oral cancer KB cells. Inhibition of SOCS1 expression in vitro significantly reduced the invasiveness of cells (P <0.05), and the number of KB cells was significantly decreased 72 h after transfection compared with the control group (P <0.05); interfere SOCS1 gene expression in KB cells, G0 / G1 phase cells (P <0.05), while the number of cells in S, G2 / M phase was significantly less than that in control group (P <0.05). CONCLUSION: After the SOCS1 gene expression in human KB cell line is disturbed, the KB cell proliferation, in vitro invasive ability weakened and cell division inhibited.