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作者构建了3种携有修饰的ctxB基因的质粒:pML-LCTBtacA、pML-LCTBtacB和pML-LCTBtacH,并分别在霍乱弧菌JS1569中表达出3种杂合蛋白:LCTBA、LCTBB和LCTBH。LCTBA的前25个氨基酸残基处与产肠毒素性大肠杆菌不耐热肠毒
The authors constructed three plasmids carrying the modified ctxB gene: pML-LCTBtacA, pML-LCTBtacB and pML-LCTBtacH, and expressed three hybrid proteins: LCTBA, LCTBB and LCTBH respectively in V. cholerae JS1569. The first 25 amino acid residues of LCTBA are not heat-tolerant enterotoxigenic to enterotoxigenic Escherichia coli