以固体豆粕为氮源,通过单因素、Plackett-Burman试验设计和正交试验,从无机氮源、胰蛋白胨、初始pH值、接种量和发酵周期方面优化了中华根霉12#产纤溶酶的液体发酵条件。试验结果表明,该菌株液体发酵产纤溶酶的适宜培养基组成为:麸皮水5%,豆粕粉6%,NaNO30.2%,NH4Cl0.1%,胰蛋白胨2.5%,初始pH4.3;适宜的培养条件是:接种量20%,发酵周期60h,纤溶酶酶活力为182.00U/mL。 Based on the single factor, Plackett-Burman experimental design and orthogonal experiment, solid-state soybean meal was used as nitrogen source to optimize 12 # fibronectin production from inorganic nitrogen source, tryptone, initial pH value, inoculum size and fermentation period Liquid fermentation conditions. The results showed that the suitable culture medium of fibrinolytic enzyme was 5% of bran water, 6% of soybean meal, 30.2% of NaNO, 0.1% of NH4Cl, 2.5% of tryptone and the initial pH of 4.3. Suitable culture conditions are: inoculum size 20%, fermentation period 60h, plasmin activity 182.00U / mL.