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目的了解河南省伤寒、副伤寒沙门菌主要流行菌型。方法用脉冲场凝胶电泳(PFGE)进行分型,NTSYSpc 2.1软件聚类分析。结果来自河南省两个监测点的伤寒、副伤寒沙门菌6株分离菌,根据XbaI酶切PFGE基因指纹图谱分析6,株菌出现5个PFGE基因型别:甲、乙副伤寒沙门菌,伤寒沙门菌3个PFGE型别。结论明确了2005年河南局部伤寒沙门菌流行菌型为X1、X2、X3三个基因型别,副伤寒沙门菌流行菌型有X4、X5基因型。
Objective To understand the major epidemic strains of Salmonella typhi in Henan Province. Methods Pulsed-field gel electrophoresis (PFGE) was used for typing and NTSYSpc 2.1 software clustering analysis. Results Six isolates of typhoid and paratyphoid Salmonella were collected from two monitoring sites in Henan Province. According to the fingerprint analysis of PFGE gene by XbaI, five PFGE genotypes were found: Salmonella typhi and Salmonella typhi, Salmonella 3 PFGE type. Conclusion The epidemic strains of Salmonella typhi in Henan Province in 2005 were identified as X1, X2 and X3 genotypes, and the genotypes of X4 and X5 were identified as Salmonella paratyphi strains.