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对沙门氏菌肠炎快速诊断，采用聚合酶链反应和ＤＮＡ序列分析，以沙门氏菌保守序列——侵袭基因Ａ作为靶序列，检测２３株沙门氏菌均扩增出２８４ｂｐ特异的ＤＮＡ片段，提示存在侵袭基因Ａ，２０株非沙门氏菌扩增阴性。检测８４份粪标本，１３份阳性，其中仅８份大便培养阳性。ＤＮＡ序列分析证实粪标本扩增出的２８４ｂｐＤＮＡ片段与侵袭基因Ａ序列一致。结果表明聚合酶链反应用于沙门氏菌肠炎诊断，具有简便、快速、敏感等优点，适于临床快速诊断
Rapid detection of Salmonella enteritis, polymerase chain reaction and DNA sequence analysis, Salmonella conserved sequence - invasion gene A as a target sequence detection of 23 strains of Salmonella amplified 284bp specific DNA fragments, suggesting the presence of invasion gene A, 20 Strain non-Salmonella amplification negative. 84 fecal specimens were tested, 13 were positive, of which only 8 were stool culture positive. DNA sequence analysis confirmed that the 284 bp DNA fragment amplified from the fecal sample was identical to the sequence of the invasive gene. The results showed that polymerase chain reaction for the diagnosis of Salmonella enteritis, with the advantages of simple, rapid, sensitive, suitable for rapid clinical diagnosis