重复序列在真核生物基因组中普遍存在,且通常是不编码蛋白质的,其主要功能尚不清楚。本文用DNA重组和PCR的方法研究了恶性疟原虫692bp中度重复序列片段的特性。 以烛缸法培养恶性疟原虫哥伦比亚FCB—2株,从非同步生长的原虫中分离DNA。自恶性疟原虫DNA文库中选出1.4kbp片段(PFCOL692),经EcoRI完全消化后,克隆至质粒pUC18中,然后导入大肠杆菌JM83中。DNA抽提并纯化后,在硝酸纤 Repeats are ubiquitous in the eukaryotic genome, and usually do not encode proteins, whose main function is not clear. In this paper, the characteristics of the 692 bp moderate repeat fragment of Plasmodium falciparum were studied by DNA recombination and PCR. The FCB-2 strain of Plasmodium falciparum was cultured by candle jar method and the DNA was isolated from non-synchronous protozoa. A 1.4 kbp fragment (PFCOL692) was selected from the P. falciparum DNA library, completely digested with EcoRI, cloned into plasmid pUC18 and then introduced into E. coli JM83. After DNA extraction and purification, the nitrocellulose