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目的研究转bar基因油菜喂饲对大鼠主要营养素(蛋白质、脂肪、粗纤维、钙)吸收利用的影响。方法将140只健康SPF级Wistar大鼠按体重随机分为7组,即饲养对照(常规基础饲料)组和低(2.5%)、中(5%)、高剂量(10%)转基因组及低(2.5%)、中(5%)、高剂量(10%)亲本组,每组20只,雌雄各半。采用喂饲方式进行染毒,连续喂饲28 d。比较各组大鼠蛋白质功效比值,蛋白质、脂肪、粗纤维、钙的表观消化率。结果与对照组比较,转基因组和亲本组的蛋白质、脂肪、粗纤维的表观消化率虽有所改变,但无剂量-反应关系和性别一致性,转基因组与亲本对照之间比较差异无统计学意义,未见其生物学意义,转基因各组和亲本各组的蛋白质功效比值和钙的表观消化率与对照组比较差异没有统计学意义。结论转bar基因油菜和亲本对照油菜“中双7号”的主要营养素(蛋白质、脂肪、粗纤维、钙)吸收利用率基本具有实质等同性。
Objective To investigate the effects of bar gene transfer to rape on the absorption and utilization of major nutrients (protein, fat, crude fiber and calcium) in rats. Methods One hundred and fourteen healthy Wistar rats were randomly divided into 7 groups according to body weight: control (conventional basal diet) and low (2.5%), moderate (5%), high dose (2.5%), medium (5%), high dose (10%) parent group, each group of 20, male and female. The animals were infected by feeding and fed for 28 days continuously. The protein efficiency, protein, fat, crude fiber and apparent digestibility of calcium in each group were compared. Results Compared with the control group, although the apparent digestibility of protein, fat and crude fiber in the transgenic group and the parental group were changed, there was no dose-response relationship and gender consistency. There was no statistical difference between the transgenic group and the parental control Significance of the study, no biological significance, no significant difference in protein efficiency ratio and apparent digestibility of calcium between the transgenic groups and the parent groups compared with the control group. Conclusion The main nutrient (protein, fat, crude fiber and calcium) absorption and utilization rate of transgenic barn and parental rape “Zhongshuang No.7” are almost the same.