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抗人肝细胞癌单克隆抗体HAb18用氯胺T法在3种不同条件下碘标记,同时用溴代琥珀酰亚胺标记。用Lindmo的双倒数作图法体外测定标记抗体~(125)I-HAb18与肝癌细胞QGY-7703结合的免疫活性分数。对不同的标记方法和标记条件下的标记参数进行比较。结果,氯胺T法在3种条件下的标记率和免疫活性分数相差不显著,免疫活性分数为0.32+0.029～0.45±0.041。而溴代琥珀酰亚胺法标记率为94.5％±3.2,免疫
The anti-human hepatocellular carcinoma monoclonal antibody HAb18 was labeled with iodine by chloramine T under three different conditions and simultaneously labeled with bromosuccinimide. The immunocompetent fraction of labeled antibody 125 I-HAb18 binding to hepatoma cells QGY-7703 was determined in vitro by double-reciprocal mapping with Lindmo. Compare different labeling methods and labeling parameters under labeling conditions. As a result, the chloramine T method showed no significant difference in the labeling rates and immunological activity scores under the three conditions, and the immunological activity fraction was 0.32 + 0.029 to 0.45 ± 0.041. The bromosuccinimide labeling method was 94.5% ± 3.2, immune