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目的探讨幽门螺旋杆菌(Hp)感染与胃息肉患者组织中低氧诱导因子-1α(HIF-1α)含量的相关性。方法胃息肉患者134例,胃癌患者73例,对照组为117例慢性胃炎患者。取胃部不同部位行改良吉姆萨染色镜检Hp;免疫组化法检测HIF-1α蛋白表达;RT-PCR和Western blotting法分别定量检测胃黏膜组织中HIF-1αm RNA和蛋白的表达水平。对3组患者Hp感染情况及HIF-1α含量进行统计分析。结果镜检Hp阳性率最高的是慢性胃炎组(80.34%),其次是胃息肉组中腺瘤性息肉(80.00%)。免疫组化结果显示HIF-1α表达阳性最高的是胃癌组和腺瘤性息肉组,分别有67例(91.78%)和4例(80.00%);RT-PCR结果示胃癌黏膜组织中HIF-1αm RNA含量最高,其次是腺瘤性息肉组;Western blotting结果显示胃癌胃黏膜组织中HIF-1α表达水平最高,其次为腺瘤性息肉组,两组相比差异有统计学意义(P<0.01)。结论胃息肉形成可能与Hp感染有关,胃息肉患者中HIF-1α蛋白表达阳性,但Hp感染与HIF-1α蛋白表达之间的关系对胃息肉的影响还有待进一步研究。
Objective To investigate the relationship between Helicobacter pylori (Hp) infection and the expression of hypoxia inducible factor-1α (HIF-1α) in gastric polyps. Methods 134 patients with gastric polyps, 73 patients with gastric cancer, the control group of 117 patients with chronic gastritis. Hp-1α protein expression was detected by modified Giemsa staining in different parts of the stomach. The expression of HIF-1αmRNA and protein in gastric mucosa were detected by RT-PCR and Western blotting respectively. Hp infection and HIF-1α levels in three groups were statistically analyzed. Results The positive rate of Hp in microscopic examination was chronic gastritis (80.34%), followed by adenomatous polyps in gastric polyps (80.00%). The results of immunohistochemistry showed that the positive expression of HIF-1αwas the highest in gastric cancer group and adenomatous polyps group, 67 cases (91.78%) and 4 cases (80.00%) respectively. RT-PCR results showed that the expression of HIF- RNA was the highest, followed by adenomatous polyps; Western blotting results showed that the expression of HIF-1αwas the highest in gastric cancer gastric mucosa tissues, followed by adenomatous polyps group, the difference was statistically significant (P <0.01) . Conclusion The formation of gastric polyps may be related to Hp infection. The expression of HIF-1α protein is positive in patients with gastric polyps. However, the relationship between Hp infection and the expression of HIF-1α protein in gastric polyps remains to be further studied.