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目的检测成纤维细胞在表皮葡萄球菌刺激下增殖及α-平滑肌肌动蛋白(α-SMA)的表达变化规律,探讨乳房假体植入术后表皮葡萄球菌感染相关包膜挛缩机制。方法 MTT法检测普通培养液、不同浓度表皮葡萄球菌SE RP62A及SE AT12228裂解液培养的成纤维细胞生长情况;Western blot及免疫组化法检测不同培养液培养成纤维细胞α-SMA的表达。结果 SE RP62A对成纤维细胞的生长有明显的促进作用(P<0.05),SE AT12228对成纤维细胞的生长无明显影响;SE RP62A能激活成纤维细胞转化为α-SMA表达阳性的肌成纤维细胞,α-SMA蛋白表达水平明显高于SE ATCC12228组及普通培养液组(P<0.01),SE ATCC12228组有部分细胞转化为肌成纤维细胞;两组细菌刺激后的细胞第5天及第7天均能维持较为稳定的α-SMA蛋白表达水平;空白组的细胞α-SMA阴性,虽然于培养第5天α-SMA蛋白表达水平有所上升,但第7天时逆转(P<0.05)。结论 SE RP62A能促进成纤维细胞增殖,相比SE ATCC12228更有利于成纤维细胞转化为肌成纤维细胞。
OBJECTIVE: To detect the proliferation and the expression of α-smooth muscle actin (α-SMA) in fibroblasts stimulated by Staphylococcus epidermidis and to investigate the mechanism of capsular contracture associated with Staphylococcus epidermidis infection after implantation of breast implants. Methods MTT method was used to detect the growth of fibroblasts cultured in normal medium, Staphylococcus epidermidis SE RP62A and SE AT22228 lysates. Western blot and immunohistochemistry were used to detect the expression of α-SMA in cultured fibroblasts. RESULTS: SE RP62A could significantly promote the growth of fibroblasts (P <0.05), while SE AT12228 had no effect on the growth of fibroblasts. SE RP62A could activate fibroblasts to transform into α-SMA positive myofibrils The expression of α-SMA protein was significantly higher than that of SE ATCC12228 group and normal medium group (P <0.01). Some cells of SE ATCC12228 group were transformed into myofibroblasts. The expression of α-SMA in the blank group was negative, although the expression of α-SMA protein increased on the 5th day of culture, but reversed on the 7th day (P <0.05) . Conclusion SE RP62A can promote the proliferation of fibroblasts, which is more conducive to the transformation of fibroblasts into myofibroblasts than SE ATCC12228.