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目的观察氧化苦参碱和槐定碱对青霉素致痫大鼠行为学和海马组织形态学的改变。方法取大鼠雌雄各半,随机分为7组,每组10只,分别为正常对照组、癫痫模型组、阳性药物苯巴比妥钠组、氧化苦参碱高剂量组、氧化苦参碱低剂量组、槐定碱高剂量组和槐定碱低剂量组。大鼠腹腔注射大剂量青霉素致痫模型,并腹腔注射氧化苦参碱和槐定碱,观察其行为学和海马组织形态学改变。结果与致痫组相比氧化苦参碱低、高剂量组和槐定碱低剂量组均能不同程度地延长痫性发作潜伏期,并且降低痫性发作程度;光镜、电镜观察使用氧化苦参碱和小剂量槐定碱后致痫大鼠海马组织神经细胞肿胀、细胞器损伤等改变得到改善。结论氧化苦参碱和小剂量槐定碱对脑组织损伤有一定的保护作用。
Objective To observe the changes of ethology and hippocampal morphological changes of oxidized matrine and sophoridine in penicillin-induced epileptic rats. Methods Male and female rats were randomly divided into 7 groups with 10 rats in each group: normal control group, epilepsy model group, positive drug phenobarbital sodium group, high-dose oxymatrine group, and oxymatrine. Low dose group, sophoridine high dose group and sophoridine low dose group. Rats were injected intraperitoneally with a large dose of penicillin-induced epilepsy model and injected with oxymatrine and sophoridine intraperitoneally to observe their behavioral and hippocampal histological changes. Results Compared with the epileptogenesis group, the low-dose oxymatrine group, the high-dose group, and the low-dose sophoridine group all prolonged the seizure latency in different degrees and reduced the degree of epileptic seizures; the use of oxymatrine was observed by light and electron microscopy. After alkali and small doses of sophoridine, the neurons in the hippocampus of rats with epileptic seizures were swollen and organelle damage was improved. Conclusion Oxymatrine and low-dose sophoridine have a protective effect on brain tissue damage.