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随着培养技术的发展,现已能使小鼠各种造血细胞亚群(Subpopulation)在半固体培养基中长成集落。目前,某些方法已用于培养人的造血细胞。可以预测,这些技术将被作为对造血紊乱病人血液学诊断与检测的常规技术。培养技术基本培养技术是将双倍浓度的组织培养基(如Dulbecco改良的Eagle培养基)和等量的双倍浓度的琼脂水溶液混合,使培养基中的最后琼脂浓度为0.3%。加入待培养的分散的骨髓或血细胞悬液,吸取1毫升上述
With the development of culture technology, it is now possible to colonize various mouse hematopoietic cell subpopulations in semi-solid medium. At present, some methods have been used to culture human hematopoietic cells. It can be predicted that these techniques will be used as a routine technique for the hematological diagnosis and detection of patients with hematopoietic disorders. Culture Techniques The basic culture technique is to mix doubled concentrations of tissue culture medium (eg, Dulbecco’s modified Eagle’s medium) and an equal amount of double strength agar solution to a final agar concentration of 0.3% in the medium. Add the suspension to be cultured to disperse bone marrow or blood cells and pipette 1 ml of the above