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在基本消灭丝虫病的广大地区,多种免疫学诊断技术已广泛地应用于流行病学监测。随之对抗原的制备及纯化提出更高的要求。为了解丝虫抗原主要蛋白成分的分子量,用SDS-聚丙烯酰胺凝胶电泳对马来丝虫成虫、微丝蚴抗原、成虫分泌代谢(E-S)抗原及牛腹腔丝虫虫体抗原进行了分析。 材料与方法 1 马来丝虫成虫及微丝蚴抗原
In the vast area of the basic elimination of filariasis, a variety of immunological diagnostic techniques have been widely used in epidemiological surveillance. Followed by the antigen preparation and purification put forward higher requirements. In order to understand the molecular weight of the major protein components of filarial antigens, adult malayian filaments, microfilariae antigens, adult secretory metabolites (ES) antigens and anti-inoculated animals were analyzed by SDS-polyacrylamide gel electrophoresis . MATERIALS AND METHODS 1 Adult Malayan filaments and microfilament antigens