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目的 检测肝细胞癌（ＨＣＣ） 患者术前血清血管内皮细胞生长因子（ＶＥＧＦ）水平与ＨＣＣ切除术后转移复发的关系。方法 采用ＥＬＩＳＡ法检测ＨＣＣ患者血清ＶＥＧＦ水平，同时应用Ｗｅｓｔｅｒｎ Ｂｌｏｔ方法对２４ 例相应ＨＣＣ组织ＶＥＧＦ表达水平进行定量分析。结果 高转移复发倾向组ＨＣＣ 患者术前血清ＶＥＧＦ水平（２８３．３３±２６３ ．１５） ｎｇ／Ｌ显著高于低转移复发倾向组（１４７．０４ ±１３２ ．６８） ｎｇ／Ｌ（ Ｐ＜ ０ ．０５） 。Ｗｅｓｔｅｒｎ Ｂｌｏｔ 显示，ＨＣＣ患者血清ＶＥＧＦ水平变化与其对应肿瘤组织ＶＥＧＦ表达变化一致。结论 ＨＣＣ患者术前血清ＶＥＧＦ水平可作为预测ＨＣＣ切除术后转移复发的指标。ＨＣＣ患者血清ＶＥＧＦ水平升高是其对应肿瘤组织高表达的结果。
Objective To detect the relationship between preoperative serum vascular endothelial growth factor (VEGF) levels and metastasis and recurrence after HCC resection in patients with hepatocellular carcinoma (HCC). Methods ELISA was used to detect serum VEGF levels in HCC patients. Western Blot was used to quantitatively analyze the expression of VEGF in 24 HCC tissues. Results The preoperative serum VEGF levels (283.33±263.15) ng/L in HCT patients with high metastasis and recurrence tendency were significantly higher than those with low metastasis and recurrence tendency (147.04±132.68) ng/L (P<0. 05). Western Blot showed that serum VEGF levels in HCC patients were consistent with their corresponding changes in VEGF expression in tumor tissues. Conclusion Preoperative serum VEGF levels in HCC patients can be used as predictors of metastasis and recurrence after HCC resection. An increase in serum VEGF levels in HCC patients is the result of high expression of the corresponding tumor tissue.