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目的探讨人类白细胞抗原(human leukocyte antigen,HLA)DP基因多态性与中国人群特发性膜性肾病(Idiopathic Membranous Nephropathy,IMN)遗传易感性的关系。方法选取185例特发性膜性肾病患者和405位健康对照作为研究对象,应用Taq Man-MGB探针基因分型方法检测HLA-DP基因多态性位点(rs3077和rs9277535)的基因型分布。结果采用多因素logistic回归分析,rs3077位点结果显示,突变基因型AA在特发性膜性肾病组中的频率均显著高于健康对照组(调整OR=1.92,95%CI=1.09-3.38);单倍型分析显示,与GG单倍型相比,携带AA单倍型的个体可增加特发性膜性肾病的患病风险;(调整OR=2.86,95%CI=1.58-5.58);此外,rs3077位点的突变基因型AA可增加特发性膜性肾病患者发生肾功能衰竭的风险(调整OR=3.11,95%CI=1.53-6.32)。结论 HLA-DP基因多态性与特发性膜性肾病遗传易感性存在关联,且与特发性膜性肾病患者的肾功能也具有相关性。
Objective To investigate the relationship between human leukocyte antigen (HLA) DP gene polymorphism and genetic susceptibility to idiopathic membranous nephropathy (IMN) in Chinese population. Methods A total of 185 patients with idiopathic membranous nephropathy and 405 healthy controls were selected as subjects. Genotype distribution of HLA-DP gene polymorphisms (rs3077 and rs9277535) was detected by TaqMan-MGB probe genotyping . Results The multivariate logistic regression analysis showed that the frequency of AA genotype in idiopathic membranous nephropathy group was significantly higher than that in healthy control group (adjusted OR = 1.92, 95% CI = 1.09-3.38) ; Haplotype analysis showed that compared with GG haplotype, individuals carrying AA haplotype increased the risk of idiopathic membranous nephropathy; (adjusted OR = 2.86, 95% CI = 1.58-5.58); In addition, mutation genotype AA at rs3077 increased the risk of renal failure in patients with idiopathic membranous nephropathy (adjusted OR = 3.11, 95% CI = 1.53-6.32). Conclusion HLA-DP gene polymorphism is associated with genetic susceptibility to idiopathic membranous nephropathy and is also associated with renal function in patients with idiopathic membranous nephropathy.