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目的探讨通过RNA干扰(RNAi)技术下调上皮性钙黏附分子(E-cad)表达后,对人卵巢浆液性囊腺癌HO-8910细胞的侵袭、迁移能力的影响。方法将针对E-cad的双链小干扰RNA(siRNA)转染入HO-8910细胞中,抑制E-cad基因表达;采用免疫荧光法及Western blotting检测RNAi效果;用Transwell小室法检测RNAi后细胞侵袭、迁移能力的改变。结果RNAi后HO-8910细胞E-cad表达显著下降,由63.7%降低为11.9%(P<0.01),RNAi有效;细胞对细胞外基质的侵袭能力明显提高,穿过滤膜的细胞数由13.4±3.93增至42.0±4.15(P<0.01);细胞迁移能力也明显提高,穿过滤膜的细胞数由23.24±3.71增至82.0±5.51(P<0.01)。结论人卵巢浆液性囊腺癌的侵袭、转移等恶性行为可能与E-cad基因表达下降有关。
Objective To investigate the effect of RNA interference (RNAi) on the invasion and migration of human ovarian serous cystadenocarcinoma HO-8910 cells after down-regulating the expression of E-cadherin. Methods Double-stranded small interfering RNA (siRNA) targeting E-cad was transfected into HO-8910 cells to inhibit the expression of E-cad gene. The effect of RNAi was detected by immunofluorescence and Western blotting. Invasion, migration ability changes. Results The expression of E-cadherin in HO-8910 cells was significantly decreased from 63.7% to 11.9% (P <0.01), and RNAi was effective. The invasion ability of cells to extracellular matrix was significantly increased, and the number of cells passing through the membrane filter increased from 13.4 ± 3.93 to 42.0 ± 4.15, respectively (P <0.01). The cell migration ability also increased obviously, and the number of cell passing through the filter increased from 23.24 ± 3.71 to 82.0 ± 5.51 (P <0.01). Conclusions The malignant behavior of human ovarian serous cystadenocarcinoma such as invasion and metastasis may be related to the decrease of E-cad gene expression.