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目的 :探讨神经肽Y和肾素 -血管紧张素系统之间的相互作用与高血压疾病发生之间的关系。方法 :应用快速微量MTT比色法和荧光免疫组化定量技术 ,观察神经肽Y对体外培养血管平滑肌细胞增殖活度和血管平滑肌细胞核细胞增殖抗原表达的影响以及血管紧张素Ⅱ受体拮抗剂losartan对其影响的干预作用。结果 :神经肽Y可刺激体外培养血管平滑肌细胞的增殖、使其增殖活度 (MTT -OD值 )和细胞内核增殖抗原(PCNA)的表达明显高于对照组 (P <0 0 1)。而losartan则可降低神经肽Y对血管平滑肌细胞增殖活度 (MTT -OD值 )的刺激作用 ,减少细胞内核增殖抗原 (PCNA)的表达。结论 :神经肽Y可促进体外培养的血管平滑肌细胞的增殖 ,并部分通过血管紧张素Ⅱ受体起作用
Objective: To investigate the relationship between neuropeptide Y and renin-angiotensin system and the occurrence of hypertension. Methods: The effects of neuropeptide Y on the proliferation activity of vascular smooth muscle cells and the expression of proliferating cell nuclear antigen of vascular smooth muscle cells in vitro were observed by rapid MTT assay and fluorescence immunohistochemical quantification. The effects of losartan The impact of its intervention. Results: Neuropeptide Y stimulated the proliferation of vascular smooth muscle cells cultured in vitro, and the proliferation activity (MTT-OD) and the expression of proliferating cell nuclear antigen (PCNA) were significantly higher than those of the control group (P <0.01). However, losartan could reduce the stimulating effect of neuropeptide Y on the proliferation activity of MTT-OD and decrease the expression of proliferating cell nuclear antigen (PCNA). Conclusion: Neuropeptide Y can promote the proliferation of vascular smooth muscle cells cultured in vitro and partly through the action of angiotensin Ⅱ receptor