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目的:研究槲皮素对心肌兴奋收缩耦联及构型重建的影响。方法:经动脉插管记录大鼠血流动力学;缩窄小鼠腹主动脉致心肌肥厚;检测Fura 2-AM负载的培养大鼠心肌细胞内游离钙([Ca~(2+)]_i)及钙振荡,结果:槲皮素剂量相关地降低大鼠窦性心率,而动脉血压、左室压及其微分改变轻微;10-250 μmol·L~(-1)时浓度依赖性降低培养心肌自发钙振荡频率,100μmol·L~(-1)时预防异丙肾上腺素及哇巴因加速钙振荡频率,但不抑制静息[Ca~(2+)]_i.和自发钙振荡及二药增高的振荡幅度,槲皮素还抑制血管紧张素Ⅱ而非高钾的升[Ca~(2+)]_i作用,槲皮素120 mg·kg~(-1)·d~(-1)5 d灌胃不改变假手术鼠的心室与体重之比,但显著抑制腹主动脉结扎小鼠的心室肥大,结论:槲皮素降低心肌钙振荡频率和心肌肥厚但不直接影响心脏兴奋收缩耦联。
Objective: To study the effect of quercetin on the excitation and contraction coupling and the conformational remodeling. Methods: The hemodynamics of rats were recorded by arterial cannulation. The cardiac hypertrophy was induced by abdominal aorta in mice. The intracellular free calcium ([Ca ~ (2 +)] i ) And calcium oscillations. Results: Quercetin dose-dependently reduced sinus rhythm in rat, while arterial blood pressure, left ventricular pressure and its differential changed slightly. In the concentration-dependent manner, 10-250 μmol·L -1 Cardiac spontaneous calcium oscillation frequency, 100μmol·L -1 prevented isoproterenol and ouabain accelerated calcium oscillation frequency, but did not inhibit resting [Ca ~ (2 +)] i. And spontaneous calcium oscillation and two Quercetin also inhibits the action of angiotensin II instead of high potassium [Ca ~ (2 +)] _ i, and the effect of quercetin is 120 mg · kg -1 · d -1 ) 5 d did not change the sham-operated rats ventricular-to-weight ratio, but significantly inhibited the abdominal aortic ligation in mice ventricular hypertrophy, Conclusion: Quercetin reduces cardiac calcium frequency and myocardial hypertrophy but does not directly affect cardiac excitatory contraction Coupling.