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目的:观察同性恋HIV感染者/AIDS患者血浆中HIV-1病毒载量、T淋巴细胞亚群变化。方法:HIV病毒载量检测采用COBASAmpliprep/COBASTaqman/COBASTaqman48 HIV,v2.0试剂盒,仪器采用COBASAmpliprep/COBASTaqman/COBASTaqman48。T淋巴细胞亚群检测采用FACSCalibur流式细胞仪,BD公司Multiset试剂盒。结果:病毒载量95.3%个体在5.1E+01拷贝·ml-1至1.0E+07拷贝·ml-1(中位数为1.5E+05拷贝·ml-1);CD3+细胞增高20例(6.26%)中有51%呈梯度减少(中位数998个·μl-1;CD4+细胞﹤400个·μl-1108例(33.85%),﹤300个·μl-1112例(35.10%),<100个·ul-169例(21.63%);CD8+细胞样本中有95%个体绝对值均在参考范围内,但其百分率均有不同程度增高;CD4+/CD8+之值除5例(1.56)>1.0外,其余均﹤1.0。临床表现无特殊。结论:319例男性同性恋HIV感染/AIDS患者的病毒载量、T淋巴细胞亚群变化及临床表现无异于其他感染途径者;CD8+细胞百分率意义大于绝对值。HIV-1分型是同性恋病毒分子流行病学的着眼点。
Objective: To observe the change of plasma HIV-1 viral load and T lymphocyte subsets in homosexual HIV / AIDS patients. Methods: The detection of HIV viral load using COBASAmpliprep / COBASTaqman / COBASTaqman48 HIV, v2.0 kit, the instrument using COBASAmpliprep / COBASTaqman / COBASTaqman48. T lymphocyte subsets were detected using FACSCalibur flow cytometry, BD Multiset kit. Results: The average viral load of 95.3% was 5.1E + 01 copies · ml-1 to 1.0E + 07 copies · ml-1 (median 1.5E + 05 copies · ml-1) (Median 998 μl-1; CD4 + cells <400 μl-1108 cases (33.85%), <300 μl-1112 cases (35.10% 100 · ul-169 cases (21.63%); 95% of CD8 + cells were in the reference range, but their percentages were all increased to a certain extent; The values of CD4 + / CD8 + , The others were all less than 1.0. The clinical manifestations were no different.Conclusion: The viral load, T lymphocyte subsets and clinical manifestations of 319 homosexual HIV / AIDS patients were no different from those of other routes of infection. The percentage of CD8 + cells was greater than The absolute value of HIV-1 typing is the focus of molecular epidemiology of homosexual viruses.