AIM: To establish nude mouse human gastric cancer orthotopic transplantation models using OB glue paste technique.METHODS: Using OB glue paste technique,orthtopic transplantation models were established by implanting SGC-7901 and MKN-45 human gastric cancer cell strains into the gastric wall of nude mice.Biological features,growth of the implanted tumors,the success rate of transplantation and the rate of auto-metastasis of the two models were observed.RESULTS: The success rates of orthotopic transplantation of the two models were 94.20% and 96%.The rates of hepatic metastasis,pulmonary metastasis,peritoneal metastasis,lymphocytic metastasis and splenic metastasis were 42.13% and 94.20%,48.43% and 57.97%,30.83% and 36.96%,67.30% and 84.06%,and 59.75% and 10.53%,respectively.The occurrence of ascites was 47.80% and 36.96%.CONCLUSION: OB glue paste technique is easy to follow.The biological behaviors of the nude mouse human gastric cancer orthotopic transplantation models established with this technique are similar to the natural processes of growth and metastasis of human gastric cancer,and,therefore,can be used as an ideal model for experimental research of proliferative metastasis of tumors.