论文部分内容阅读
为了探索云南省高原粳稻上十种不同致病型的白叶枯病菌对噻枯唑、叶枯灵和新植霉素三种农药的抗药性及其机制,分别用含有不同浓度农药的NA培养基进行白叶枯病菌的室内抗药性筛选,并设计与菌株抗药性密切相关的rpfC基因特异引物,对抗药性不同的菌株进行扩增、测序、基因和氨基酸序列比对分析。结果表明,噻枯唑对参试的所有菌株的最小抑菌浓度(MIC)为40~180mg/L,而叶枯灵为10~100mg/L,没有发现对新植霉素产生抗性的菌株;病原菌的致病型与对农药的敏感性相关,致病性强的菌株,其抗药性较强。将致病型和抗药性不同的10个菌株的rpfC基因序列与GenBank中登录号为X97865.1的基因序列比对,序列同源性为92%~98%,而RpfC蛋白序列同源性差异较大(8.3%至99%)。致病性和抗药性最强的Ⅵ型菌株2001-31的RpfC蛋白序列的六个功能域完整,致病性和抗药性最弱的0型菌株DH-L-1的RpfC蛋白序列的信号接收区域REC已经消失。7个致病性和抗药性中等的菌株中,Ⅳ型菌株5较为特殊,其RpfC蛋白序列已经不能形成功能域。
In order to explore the resistance and mechanisms of three isolates of thiophenezole, levofloxacin and neomycin with three pathogenic isolates of Xanthomonas campestris on Yunnan plateau rice, NA were cultured in NA with different concentrations of pesticides Based on the rpfC gene-specific primers closely related to the resistance of the strains, the strains with different drug resistances were amplified, sequenced, and the sequences of the genes and amino acid sequences were compared. The results showed that the minimum inhibitory concentration (MIC) of all the tested strains was 40 ~ 180mg / L for thiophene and 10 ~ 100mg / L for Yeku Ling, and no strains resistant to new phytocidal were found ; Pathogenic type of pathogenic bacteria and the sensitivity of pesticides, pathogenic strains, its strong resistance. The rpfC gene sequences of 10 strains with different pathogenicity and drug resistance were compared with the GenBank accession number X97865.1, the sequence homology was 92% -98%, while the RpfC protein sequence homology was different Larger (8.3% to 99%). The six pathways of the RpfC protein sequence of type VI strain 2001-31 with the strongest pathogenicity and drug resistance The signal integrity of the intact, pathogenic and drug-resistant strain of type 0 DH-L-1 RpfC protein The area REC has disappeared. Of the seven pathogenic and drug-resistant isolates, type IV strain 5 was more specific and its RpfC protein sequence no longer formed a functional domain.