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目的 分离与胃癌发生相关的基因片段并予以鉴定。方法 运用单引物AP -PCR技术 ,对 2 0例配对的胃癌组织和非癌胃组织的基因DNA进行扩增 ,分离出差异片段 ,进行克隆 ,测序和杂交鉴定。结果 分离出三条差异片段 ,其中一条片段 ( 8ndf)基因文库内无同源序列存在 ,斑点杂交显示该片段存在于非癌组织中而癌组织中缺如。结论 单引物AP -PCR技术对于分离差异基因行之有效 ;该片段可能为新的候选抑癌基因
Objective To isolate and identify the gene fragments associated with gastric cancer. METHODS: A pair of paired gastric cancer tissues and non-cancer gastric tissue DNA was amplified by single primer AP-PCR technique. The differential fragments were isolated and cloned, sequenced and identified. Results Three different fragments were isolated. One of the fragments (8ndf) had no homologous sequences in the library. Spot hybridization showed that the fragments existed in non-cancer tissues and were absent in cancer tissues. Conclusion The single-primary AP-PCR technique is effective for isolating differential genes; this fragment may be a new candidate tumor suppressor gene