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目的:通过环磷酰胺对大鼠睾丸组织中干细胞因子的表达及增殖指数影响的研究,以探讨环磷酰胺所致生精功能损害的原因。方法:选取9天龄雄性Wistar大鼠,腹腔注射大、中、小剂量的环磷酰胺后24h、4w、8w分别采集睾丸标本,应用RT-PCR技术检测睾丸组织中SCF的表达,同时光镜下计数4w时睾丸组织的增殖指数。结果:(1)对照组随年龄的增加,睾丸组织中分泌型干细胞因子表达无显著差异(P>0.05),膜型干细胞因子均有显著增加(P<0.05)。(2)同一时期各实验组和对照组比较,分泌型干细胞因子表达无显著差异(P>0.05),但膜型干细胞因子表达均有显著降低(P<0.05);同一时期各实验组间比较,随环磷酰胺剂量增加,膜型干细胞因子表达有显著降低(P<0.05)。(3)同一剂量三个时期比较,各实验组分泌型干细胞因子无显著差异(P>0.05);膜型干细胞因子比较,大剂量组中无显著差异(P>0.05),中、小剂量组中24h较4w、8w均有显著降低(P<0.05),4w与8w比较无显著差异(P>0.05)。(4)增殖指数检测,4w时各实验组与对照组比较,均有显著降低(P<0.01),并与剂量负相关。结论:本研究结果提示由于环磷酰胺的毒性作用,导致了睾丸组织中膜型干细胞因子表达下调,进而影响增殖指数降低,这可能即是临床上环磷酰胺使用后产生不同程度生精功能障碍的原因之一。
OBJECTIVE: To investigate the effect of cyclophosphamide on the expression of stem cell factor and proliferation index in rat testis to investigate the causes of spermatogenic dysfunction induced by cyclophosphamide. Methods: Nine-day-old male Wistar rats were injected intraperitoneally with large, medium and small doses of cyclophosphamide. The specimens of testis were collected at 24h, 4w and 8w respectively. The expression of SCF in testis was detected by RT-PCR. Under the count 4w testis tissue proliferation index. Results: (1) There was no significant difference in the expression of secretory stem cell factor between the testis group and the control group (P> 0.05). The membrane type of stem cell factor increased significantly (P <0.05). (2) Compared with the control group, there was no significant difference in the expression of secretory stem cell factor between the experimental group and the control group in the same period (P> 0.05), but the expression of membrane-derived stem cell factor was significantly decreased (P <0.05) , With the increase of dose of cyclophosphamide, membrane-derived stem cell factor expression was significantly reduced (P <0.05). (3) There was no significant difference of secreting type of stem cell factor between the three groups at the same dose (P> 0.05). There was no significant difference between the high dose group and the membrane type of stem cell factor (P> 0.05) (P <0.05), but there was no significant difference between 4w and 8w (P> 0.05). (4) Proliferation index detection, 4w each experimental group compared with the control group, were significantly lower (P <0.01), and dose-related negative. Conclusion: The results of this study suggest that due to the toxic effects of cyclophosphamide, the expression of membrane-derived stem cell factor in testis tissue is down-regulated and thus the proliferation index is decreased. This may be the clinical use of cyclophosphamide to produce different degrees of spermatogenesis dysfunction One of the reasons.