论文部分内容阅读
背景:丙二醛是脂质过氧化的终产物,丙二醛含量可以推断机体内的脂质过氧化损伤情况;谷胱甘肽过氧化物酶是生物机体内的自由基清除剂。而稳恒磁场对生物体的正、负面效应目前尚无定论。目的:探讨稳恒磁场对小鼠肝脏组织抗氧化能力的影响及其强度效应。设计:观察对比实验。单位:江苏大学医学物理实验室、生化实验室。材料:实验于2003-01/12在江苏大学医学物理实验室及生化实验室完成。选择昆明种小鼠30只,雌雄各半,体质量18～20g。暴露装置采用铁氧体瓦形磁铁自制暴露盒。方法:将30只小鼠随机分成5组,正常对照组、磁感应强度(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT组各6只。每天定时将正常对照组小鼠放入无磁场暴露盒内,磁感应强度(24.6±4.2),(42.0±2.1),(63.5±3.0),(85.1±2.9)mT组小鼠分别置于以上4种不同强度的稳恒磁场暴露盒内2次,2h/次,15d后麻醉处死小鼠,检测肝脏组织中谷胱甘肽过氧化物酶活性及丙二醛含量。主要观察指标:各组小鼠肝脏组织中丙二醛含量及谷胱甘肽过氧化物酶活性。结果:30只小鼠全部进入结果分析,无脱失。①小鼠肝脏组织中丙二醛含量:磁感应强度(24.6±4.2)mT,(42.0±2.1)mT组小鼠显著低于正常对照组[(12.70±0.53,12.96±0.72,17.62±0.91)μmol/g(F=10.4,9.89,P<0.01)]。②小鼠肝脏组织中谷胱甘肽过氧化物酶活性:磁感应强度(24.6±4.2)mT,(42.0±2.1)mT组小鼠显著高于正常对照组[(143.36±8.34,150.69±12.00,87.51±11.34)μkat/g(F=10.0,11.3,P<0.01)]。结论:一定磁感应强度的稳恒磁场能降低小鼠肝组织的丙二醛含量,增加谷胱甘肽过氧化物酶活性,提高抗氧化酶的活力,降低过氧化脂质的生成,减少其对生物体的损害,对延缓衰老有积极作用。
BACKGROUND: Malondialdehyde (MDA) is the end product of lipid peroxidation. Malondialdehyde (MDA) content can infer lipid peroxidation damage in the body. Glutathione peroxidase is a free radical scavenger in biological tissues. However, the positive and negative effects of the steady magnetic field on the organism are not conclusive at present. Objective: To investigate the effect of steady magnetic field on anti-oxidative ability of liver tissue and its intensity effect. Design: observe the contrast experiment. Unit: Jiangsu Medical Physics Laboratory, Biochemical Laboratory. Materials: The experiment was performed at Medical Physics Laboratory and Biochemical Laboratory, Jiangsu University from January to December in 2003. Select Kunming mice 30, male and female, body weight 18 ~ 20g. The exposure device uses a ferrite tile-shaped magnet to make a self-made exposure box. Methods: Thirty mice were randomly divided into five groups: normal control group, magnetic induction intensity (24.6 ± 4.2), (42.0 ± 2.1), (63.5 ± 3.0), and (85.1 ± 2.9) mT group. The mice in normal control group were placed in non-magnetic field exposure box at regular time every day. The mice with magnetic induction intensity (24.6 ± 4.2), (42.0 ± 2.1), (63.5 ± 3.0) and (85.1 ± 2.9) mT were placed in the above 4 The mice were sacrificed 2h, 2h, 15h later after exposure to magnetic field of different intensity. The activities of glutathione peroxidase and malondialdehyde in liver tissue were measured. MAIN OUTCOME MEASURES: Malondialdehyde content and glutathione peroxidase activity in liver tissues of mice in each group. Results: All 30 mice entered the result analysis without loss. (1) The content of malondialdehyde in liver tissue of mice: the magnetic induction intensity (24.6 ± 4.2) mT, (42.0 ± 2.1) mT group was significantly lower than that of the normal control group [(12.70 ± 0.53,12.96 ± 0.72,17.62 ± 0.91) μmol /g(F=10.4,9.89,P<0.01)]. (2) The glutathione peroxidase activity in mouse liver tissue: The magnetic induction intensity (24.6 ± 4.2) mT, (42.0 ± 2.1) mT group was significantly higher than that in the normal control group [(143.36 ± 8.34,150.69 ± 12.00,87.51 ± 11.34) μkat / g (F = 10.0, 11.3, P <0.01)]. CONCLUSION: The steady magnetic field with certain magnetic induction can reduce the content of malondialdehyde, increase the activity of glutathione peroxidase, increase the activity of antioxidant enzyme, decrease the production of lipid peroxidation, Damage to living organisms has a positive effect on anti-aging.