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采用酶学和逆转录聚合酶链式反应 ( RT-PCR)技术 ,研究与药物代谢密切相关的细胞色素P450 3A( CYP3A)在胎肾上腺和胎肝中的表达 .结果表明 ,胎肾上腺微粒体中苄非他明 ,氨基比林 ,红霉素 N-脱甲基酶和睾酮 6β-羟化酶活性分别为胎肝微粒体的 2 1 % ,2 60 % ,1 0 5%和 33% .CYP诱导剂苯巴比妥能显著增加体外培养胎肾上腺细胞中苄非他明和氨基比林 N-脱甲基酶活性 ,诱导剂地塞米松也能明显诱导红霉素 N-脱甲基酶活性 .RT- PCR表明胎肾上腺和胎肝中存在 CYP3A7m RNA表达 .说明胎肾上腺和胎肝中存在 CYP3A亚族 .上述结果提示处于发育时期的肾上腺具有与胎肝能力相当的药物代谢功能 .
The expression of cytochrome P450 3A (CYP3A), which is closely related to drug metabolism, in fetal adrenal gland and fetal liver was studied by enzymology and reverse transcriptase polymerase chain reaction (RT-PCR) .Results showed that in fetal fetal microsome Benzofiracetam, aminopyrine, erythromycin N-demethylase and testosterone 6β-hydroxylase activity were 21%, 260%, 105% and 33% of fetal liver microsomes, respectively.CYP Induction agent phenobarbital can significantly increase fetal bovine adrenal cells cultured in vitro amphetamine and aminopyrine N-demethylase activity inducer dexamethasone can also significantly induce erythromycin N-demethylase activity. RT-PCR showed CYP3A7m RNA expression in fetal adrenal gland and fetal liver, indicating that there is CYP3A subfamily in fetal adrenal and fetal liver.The above results suggest that the adrenal gland in development has the same drug metabolism function as fetal liver.