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从桃仁提得总蛋白组分,用聚丙烯酰胺凝胶电泳分析,显示主要由6条蛋白区带组成。用凝胶过滤柱层析法,从桃仁总蛋白分得二个均一蛋白成分,组分F和组分G,等电点分别为4.7～5.1和4.1～4.7,分子量分別为257,000和19,000(SDS-PAGE法)。组分F含有18种氨基酸,组分G含17种氨基酸,其中谷氨酸含量为41.69%。这二种蛋白成分,ⅳ给药,对二甲苯所致小鼠耳部急性炎症反应,均有显著抑制作用。
Total protein fractions were extracted from peach kernels and analyzed by polyacrylamide gel electrophoresis and showed that they consisted mainly of six protein bands. By gel filtration chromatography, two homogenous protein fractions were obtained from the total protein of the peach kernel, component F and component G, with isoelectric points of 4.7 to 5.1 and 4.1 to 4.7, respectively, and molecular weights of 257,000 and 19,000 (SDS), respectively. -PAGE method). Component F contains 18 amino acids, and Component G contains 17 amino acids, of which the glutamic acid content is 41.69%. These two protein components, iv administration, have significant inhibitory effects on acute inflammatory reactions in the ear of mice induced by xylene.