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目的:从基因水平探讨内皮素(ET)在运动性心脏重塑过程中的作用。方法:将70只SD大鼠随机分为对照组和实验组,每组35只。实验组进行75天的跑台耐力训练。分别于训练第3、10、23、37和75天次日晨宰杀实验组大鼠,每次7只。相应对照组亦于当日上午宰杀取材。以异硫氰酸胍法提取大鼠心室肌组织总RNA。采用反转录聚合酶链式反应(RT-PCR)技术,测定大鼠心室肌中ET-1mRNA的表达量,β-actin为内参照。ET-1mRNA/β-actinmRNA的比值为每个测试样本中ET-1mRNA的表达量。结果:第23天时,实验组大鼠心室肌组织中的ET-1mRNA表达量显著高于对照组(P<0.05),其余4个时相点二者均无显著性差异。结果提示:运动性心脏重塑过程中,大鼠心室肌组织ET-1mRNA表达量可能以上调的方式参与心脏结构与功能的重塑,运动适应后其表达量恢复至训练前水平。
Objective: To explore the role of endothelin (ET) in the process of exercise-induced cardiac remodeling at the gene level. Methods: Seventy SD rats were randomly divided into control group and experimental group, with 35 rats in each group. The experimental group carried out 75 days treadmill training. Rats in experimental group were sacrificed on the morning of the 3rd, 10th, 23th, 37th and 75th day of training respectively, each time 7 rats. The corresponding control group also slaughtered the same day in the morning. The total RNA of ventricular myocytes was extracted by guanidine isothiocyanate method. RT-PCR was used to determine the expression of ET-1 mRNA in rat ventricular muscle. Β-actin was used as an internal control. The ratio of ET-1 mRNA / β-actin mRNA is the expression level of ET-1 mRNA in each test sample. Results: On the 23rd day, the expression of ET-1mRNA in the ventricular muscle tissue of the experimental group was significantly higher than that of the control group (P <0.05). There was no significant difference between the other four time points. The results suggest that ET-1mRNA expression in rat cardiac ventricular myocytes may be involved in the remodeling of cardiac structure and function in exercise-induced cardiac remodeling. After exercise adaptation, the expression level of ET-1mRNA may be restored to the pre-training level.