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以8个导入了空心莲子草DNA的水稻导入系和2个对照品种(原受体6527和巴西陆稻)为材料,采用裂区试验设计,在干旱胁迫条件下测定其剑叶的超氧化物歧化酶、丙二醛、电导率、脯氨酸、叶绿素a、叶绿素b和总叶绿素含量等生理指标,并进行主成分分析和逐步回归分析。结果表明,导入系H8的产量、综合评价值和耐旱指数明显大于对照,其耐旱性强。主成分分析和最优回归方程分析均表明丙二醛、脯氨酸、叶绿素和电导率等对水稻耐旱性有显著影响,可作为水稻耐旱性生理评价的重要参考指标。
Eight rice introduced with Alternanthera philoxeroides and two control cultivars (original receptor 6527 and Brazilian upland rice) were used as experimental materials to study the effects of drought stress on the superoxide dismutase MDA, MDA, proline, chlorophyll a, chlorophyll b and total chlorophyll content were determined and analyzed by principal component analysis and stepwise regression analysis. The results showed that the yield, comprehensive evaluation index and drought tolerance index of H8 were significantly higher than that of the control, and their drought tolerance was strong. Principal component analysis and regression analysis showed that MDA, proline, chlorophyll and conductivity had a significant effect on drought tolerance of rice, which could be used as an important reference index for physiological evaluation of drought tolerance in rice.