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目的在构建 THANK 腺病毒载体的基础上,在裸鼠体内观察 THANK 基因转移对SMMC-7721细胞作用。方法用 THANK 重组腺病毒,感染 SMMC-7721细胞,获取高表达THANK 基因的7721细胞,将不同7721细胞接种裸鼠,观察 THANK 基因转移后7721细胞的成瘤性变化,包括肿瘤生长的大小、速率,肿瘤重量、裸鼠生存期变化以技成瘤后肿瘤组织及脾脏的微观结构变化。结果 THANK 在7721细胞中的表达可抑制肿瘤细胞的生长,激发机体的抗肿瘤免疫,抑制初次和再次接种的亲代7721细胞的生长,降低了7721细胞的成瘤性。光镜和电镜观察均可发现THANK 的转染使得7721细胞的坏死和凋亡比例有所增高,提示 THANK 在裸鼠体内可能通过某种机制增强了7721细胞的凋亡比例。结论 THANK 在裸鼠体内可诱发有效的抗肿瘤免疫。
Objective To construct a THANK adenovirus vector and observe the effect of THANK gene transfer on SMMC-7721 cells in nude mice. Methods THANK recombinant adenovirus was used to infect SMMC-7721 cells to obtain 7721 cells with high expression of THANK gene. The different 7721 cells were inoculated with nude mice to observe the tumorigenicity of 7721 cells after THANK gene transfer, including the size and rate of tumor growth , Tumor weight, changes in the survival of nude mice after tumor formation and spleen microstructure changes. Results The expression of THANK in 7721 cells inhibited the growth of tumor cells, stimulated the body’s anti-tumor immunity, inhibited the growth of primary and secondary inoculated parental 7721 cells, and reduced the tumorigenicity of 7721 cells. Both light and electron microscopy showed that THANK transfection increased the percentage of necrosis and apoptosis in 7721 cells, suggesting that THANK may enhance the apoptosis rate of 7721 cells in some ways in nude mice. Conclusion THANK can induce effective anti-tumor immunity in nude mice.