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为鉴定新疆某些地区鸡白痢沙门氏菌主要流行株的流行现状和耐药情况,本研究采用平板凝集试验方法对有发病史的种鸡场进行现场检测,被检的750只鸡中,发现78例疑似鸡白痢沙门氏菌感染,运用沙门氏菌鉴别培养基、组织病理学观察和PCR方法进行确诊,并进行药敏试验。结果显示,疑似的78例鸡白痢沙门氏菌中有69例符合鸡白痢沙门氏菌的生化特性和病理学特点,PCR成功扩增出invA基因,分离率为9.2%,血清型诊断为D1型;其中分离菌对万古霉素和新霉素耐药性最高,耐药率分别为100.0%和81.2%,而对头孢曲松、克拉维酸和多黏菌素的敏感率达60.0%以上。本研究为养殖户和兽医工作者在净化鸡白痢沙门氏菌及临床用药方面提供参考。
In order to identify the epidemic situation and drug resistance of Salmonella pullorum in some areas of Xinjiang, this study used the plate agglutination test method to test the breeder farms with the history of onset. Among the 750 chickens examined, 78 cases Susceptible Salmonella pullorum infection, the use of Salmonella identification medium, histopathological observation and PCR method for diagnosis, and susceptibility testing. The results showed that 69 cases of Salmonella pullorum were suspected to be biochemical and pathological characteristics of Salmonella pullorum. The invA gene was amplified by PCR, the isolation rate was 9.2% and the serotype was diagnosed as D1 type. Among them, The highest rates of resistance to vancomycin and neomycin were 100.0% and 81.2%, respectively, while those of ceftriaxone, clavulanic acid and polymyxin were above 60.0%. This study provides reference for farmers and veterinary workers to purify Salmonella pullorum and clinical use.