论文部分内容阅读
目的:制备杨梅苷脂质体。方法:采用逆相蒸发法制备杨梅苷脂质体。用冷冻离心法分离脂质体和游离药物,用高效液相色谱法测定药物含量并计算包封率。采用激光粒度仪测定平均粒径。结果:杨梅苷脂质体制备的最佳处方和工艺为:卵磷脂:杨梅6:1,卵磷脂:胆固醇2:1,有机相:水相4:1,磷酸盐缓冲溶液的pH值为6.86,浓度为0.005 mol.L-1;超声时间为5分钟。结论:最佳条件下制备的杨梅苷脂质体包封率较高,粒径分布好,质量稳定。
Objective: To prepare myricetin liposomes. Methods: Myricetin liposomes were prepared by reverse phase evaporation. Liposomes and free drug were separated by freeze-centrifugation, drug content was determined by high performance liquid chromatography and encapsulation efficiency was calculated. The average particle size was measured by laser particle sizer. Results: The optimum formulation and process of myricline liposome preparation were as follows: lecithin: bayberry 6: 1, lecithin: cholesterol 2: 1, organic phase: water phase 4: 1, pH value of phosphate buffer solution was 6.86 , The concentration of 0.005 mol.L-1; ultrasonic time of 5 minutes. CONCLUSION: Myricetin liposome prepared under optimal conditions has high encapsulation efficiency, good particle size distribution and stable quality.