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目的:了解铅接触及铅中毒工人外周血中T细胞抗原受体(TCR)的Vγ基因表达水平。方法:利用SYBR GreenⅠ荧光定量PCR分别检测12例铅接触工人、8例铅中毒工人和20例正常人外周血单个核细胞中TCRVγ基因表达情况,以β2微球蛋白基因(β2M)作为内参,根据相对定量公式(2-△Ct×100%)计算铅接触、铅中毒工人与正常人的TCR VγⅠ、TCR VγⅡ和TCR VγⅢ基因表达水平差异。结果:正常人、铅接触和铅中毒工人外周血的TCR VγⅠ基因表达水平的中位数分别为0.79%、1.22%和0.96%;TCR VγⅡ基因表达水平分别为0.94%、1.26%和2.74%;TCR VγⅢ基因表达水平分别为0.46%、0.43%和1.04%。与正常人相比,铅中毒工人的TCRVγⅡ基因表达显著上升(P<0.05)。结论:铅接触及铅中毒工人中外周血TCRVγ基因表达异常。
Objective: To understand the expression of Vγ gene in T cell receptor (TCR) in peripheral blood of lead exposed and lead poisoned workers. Methods: The expression of TCRVγ gene in peripheral blood mononuclear cells from 12 lead exposed workers, 8 lead poisoned workers and 20 normal controls were detected by SYBR Green Ⅰ fluorescent quantitative PCR. Β2 microglobulin gene (β2M) The relative quantification formula (2- △ Ct × 100%) was used to calculate the difference of TCR VγⅠ, TCR VγⅡ and TCR VγⅢ gene expression between lead poisoning workers and normal people. Results: The median of TCR Vγ Ⅰ gene expression in peripheral blood of workers exposed to lead and lead poisoned were 0.79%, 1.22% and 0.96% respectively, and the levels of TCR VγⅡ gene were 0.94%, 1.26% and 2.74% respectively. TCR VγⅢ gene expression levels were 0.46%, 0.43% and 1.04%, respectively. Compared with normal controls, TCRVγⅡgene expression was significantly increased in lead poisoning workers (P <0.05). Conclusion: The abnormal expression of TCRVγ gene in peripheral blood of lead exposure and lead poisoning workers.