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利用壳聚糖固定化胰蛋白酶作为亲和吸附剂从牛肺中直接分离纯化抑防酶。该方法在适当的温度（35℃）、pH值（pH1．5）、离子强度（1．0mol／LKCI）以及吸附条件（176FIP／g）下，纯化的抑肽酶活性回收率达83％，比活可达5000kIU／mg以上。
Chitosan immobilized trypsin was used as an affinity adsorbent to directly separate and purify inhibitory enzymes from bovine lung. The method can recover 83% of the purified aprotinin at the proper temperature (35 ℃), pH value (pH1.5), ionic strength (1.0mol / L KCI) and adsorption condition (176FIP / g) Specific activity up to 5000kIU / mg or more.